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 | Acesso ao texto completo restrito à biblioteca da Embrapa Uva e Vinho. Para informações adicionais entre em contato com cnpuv.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
03/01/2019 |
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Data da última atualização: |
27/04/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VALENTE, J. B.; PEREIRA, F. S.; STEMPKOWSKI, L. A.; FARIAS, M.; KUHNEM, P.; LAU, D.; FAJARDO, T. V. M.; NHANI JUNIOR, A.; CASA, R. T.; BOGO, A.; SILVA, F. N. da. |
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Afiliação: |
J. B. VALENTE, Crop Production Graduate Program, Santa Catarina State University/UDESC, Lages, SC 88520-000; F. S. Pereira, Crop Production Graduate Program, Santa Catarina State University/UDESC, Lages, SC 88520-000; L. A. Stempkowski, Crop Production Graduate Program, Santa Catarina State University/UDESC, Lages, SC 88520-000; M. Farias, Crop Production Graduate Program, Santa Catarina State University/UDESC, Lages, SC 88520-000; P. Kuhnem, Biotrigo Gen etica, Passo Fundo, RS 99052-160; DOUGLAS LAU, CNPT; THOR VINICIUS MARTINS FAJARDO, CNPUV; ANTONIO NHANI JUNIOR, CNPTIA; R. T. CASA, Crop Production Graduate Program, Santa Catarina State University/UDESC, Lages, SC 88520-000; A. Bogo, Crop Production Graduate Program, Santa Catarina State University/UDESC, Lages, SC 88520-000; F. N. da Silva, Crop Production Graduate Program, Santa Catarina State University/UDESC, Lages, SC 88520-000. |
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Título: |
A novel putative member of the family Benyviridae is associated with soilborne wheat mosaic disease in Brazil. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Plant Pathology, First online, 2018. |
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DOI: |
10.1111/ppa.12970 |
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Idioma: |
Inglês |
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Notas: |
DIsponível em: |
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Conteúdo: |
Soilborne wheat mosaic disease (SBWMD), originally attributed to infections by Soilborne wheat mosaic virus (SBWMV) and Wheat spindle streak mosaic virus (WSSMV), is one of the most frequent virus diseases and causes economic losses in wheat in southern Brazil. This study aimed to characterize molecularly the viral species associated with wheat plants showing mosaic symptoms in Brazil. Wheat leaves and stems displaying mosaic symptoms were collected from different wheat cultivars in Passo Fundo municipality, Rio Grande do Sul State, southern Brazil. Double-stranded RNA was extracted and submitted to cDNA library synthesis and next-generation sequencing. No sequences of SBWMV and WSSMV were detected but the complete genome sequence of a putative new member of the family Benyviridae was determined, for which the name wheat stripe mosaic virus (WhSMV) is proposed. WhSMV has a bipartite genome with RNA 1 and RNA 2 organization similar to that of viruses belonging to Benyviridae . WhSMV RNA 1 has a single open reading frame (ORF) encoding a polyprotein with putative viral replicase function. WhSMV RNA 2 has six ORFs encoding the coat protein, the major protein (read-through), triple gene block movement proteins (TGB 1, 2 and 3) and ORF 6 (hypothetical protein). In addition to the genomic organization and nucleotide and amino acid sequence identi- ties, phylogenetic analyses also corroborated that WhSMV is a virus species of the Benyviridae . However, isolates of WhSMV formed a clade distinct from members of the genus Benyvirus . It was also demonstrated that the plasmodio- phorid Polymyxa graminis is associated with wheat roots showing SBWMD symptoms and infected by WhSMV. Keywords: Benyviridae , mosaic symptoms, next-generation sequencing, Polymyxa graminis , Triticum aestivum , wheat stripe mosaic virus MenosSoilborne wheat mosaic disease (SBWMD), originally attributed to infections by Soilborne wheat mosaic virus (SBWMV) and Wheat spindle streak mosaic virus (WSSMV), is one of the most frequent virus diseases and causes economic losses in wheat in southern Brazil. This study aimed to characterize molecularly the viral species associated with wheat plants showing mosaic symptoms in Brazil. Wheat leaves and stems displaying mosaic symptoms were collected from different wheat cultivars in Passo Fundo municipality, Rio Grande do Sul State, southern Brazil. Double-stranded RNA was extracted and submitted to cDNA library synthesis and next-generation sequencing. No sequences of SBWMV and WSSMV were detected but the complete genome sequence of a putative new member of the family Benyviridae was determined, for which the name wheat stripe mosaic virus (WhSMV) is proposed. WhSMV has a bipartite genome with RNA 1 and RNA 2 organization similar to that of viruses belonging to Benyviridae . WhSMV RNA 1 has a single open reading frame (ORF) encoding a polyprotein with putative viral replicase function. WhSMV RNA 2 has six ORFs encoding the coat protein, the major protein (read-through), triple gene block movement proteins (TGB 1, 2 and 3) and ORF 6 (hypothetical protein). In addition to the genomic organization and nucleotide and amino acid sequence identi- ties, phylogenetic analyses also corroborated that WhSMV is a virus species of the Benyviridae . However, isolates of WhSMV formed a cl... Mostrar Tudo |
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Palavras-Chave: |
Benyviridae; Mosaic symptoms; Next-generation sequencing; Wheat stripe mosaic virus. |
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Thesagro: |
Polymyxa Graminis; Triticum Aestivum. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02870naa a2200337 a 4500
001 2102942
005 2019-04-27
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1111/ppa.12970$2DOI
100 1 $aVALENTE, J. B.
245 $aA novel putative member of the family Benyviridae is associated with soilborne wheat mosaic disease in Brazil.$h[electronic resource]
260 $c2018
500 $aDIsponível em: <https://onlinelibrary.wiley.com/doi/epdf/10.1111/ppa.12970>
520 $aSoilborne wheat mosaic disease (SBWMD), originally attributed to infections by Soilborne wheat mosaic virus (SBWMV) and Wheat spindle streak mosaic virus (WSSMV), is one of the most frequent virus diseases and causes economic losses in wheat in southern Brazil. This study aimed to characterize molecularly the viral species associated with wheat plants showing mosaic symptoms in Brazil. Wheat leaves and stems displaying mosaic symptoms were collected from different wheat cultivars in Passo Fundo municipality, Rio Grande do Sul State, southern Brazil. Double-stranded RNA was extracted and submitted to cDNA library synthesis and next-generation sequencing. No sequences of SBWMV and WSSMV were detected but the complete genome sequence of a putative new member of the family Benyviridae was determined, for which the name wheat stripe mosaic virus (WhSMV) is proposed. WhSMV has a bipartite genome with RNA 1 and RNA 2 organization similar to that of viruses belonging to Benyviridae . WhSMV RNA 1 has a single open reading frame (ORF) encoding a polyprotein with putative viral replicase function. WhSMV RNA 2 has six ORFs encoding the coat protein, the major protein (read-through), triple gene block movement proteins (TGB 1, 2 and 3) and ORF 6 (hypothetical protein). In addition to the genomic organization and nucleotide and amino acid sequence identi- ties, phylogenetic analyses also corroborated that WhSMV is a virus species of the Benyviridae . However, isolates of WhSMV formed a clade distinct from members of the genus Benyvirus . It was also demonstrated that the plasmodio- phorid Polymyxa graminis is associated with wheat roots showing SBWMD symptoms and infected by WhSMV. Keywords: Benyviridae , mosaic symptoms, next-generation sequencing, Polymyxa graminis , Triticum aestivum , wheat stripe mosaic virus
650 $aPolymyxa Graminis
650 $aTriticum Aestivum
653 $aBenyviridae
653 $aMosaic symptoms
653 $aNext-generation sequencing
653 $aWheat stripe mosaic virus
700 1 $aPEREIRA, F. S.
700 1 $aSTEMPKOWSKI, L. A.
700 1 $aFARIAS, M.
700 1 $aKUHNEM, P.
700 1 $aLAU, D.
700 1 $aFAJARDO, T. V. M.
700 1 $aNHANI JUNIOR, A.
700 1 $aCASA, R. T.
700 1 $aBOGO, A.
700 1 $aSILVA, F. N. da
773 $tPlant Pathology, First online, 2018.
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Embrapa Uva e Vinho (CNPUV) |
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| 1. |  | KLEINERT, A. M. P.; RAMALHO, M.; CORTOPASSI-LAURINO, M.; RIBEIRO, M. de F.; IMPERATRIZ-FONSECA, V. L. Abelhas sociais (Bombini, Apini, Meliponini). In: PANIZZI, A. R.; PARRA, J. R. P. (Ed.). Bioecologia e nutrição de insetos: base para o manejo integrado de pragas. Brasília, DF: Embrapa Informação Tecnológica; Londrina: Embrapa Soja, 2009. cap. 10, p. 371-424.| Tipo: Capítulo em Livro Técnico-Científico |
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